Tuberculosis (TB) is a global emergency caused by Mycobacterium tuberculosis (MTB). One third of the world population is infected. Finding biomarker(s) for rapid diagnosis of TB becomes one of the essential requirements for TB control. Single biomarkers or combination of a few biomarkers fail in identification of MTB. Recently we have developed a fully automated GC-MS method using Thermally assisted Hydrolysis and Methylation (THM) for sputum analysis from TB-suspected
patients . In addition, using chemometrics, we found a list of 20 potential compounds giving better accuracy for diagnosis of TB. Unfortunately, the method still needs further improvement because of the adverse effect of the sputum matrix on the detection limit. Moreover, the diagnosis of TB in HIV positive patients, a high risk group for multidrug resistant TB, remains a problem because their sputum contains fewer mycobacteria than the sputum from HIV negative TB patients.
Posted: June 11, 2012, 1:50 pm
ATAS GL International B.V. has changed her name into GL Sciences B.V.. We are based in Eindhoven, The Netherlands. Founded in 1990, GL Sciences B.V specializes in development and manufacturing of...